bisulfite sequencing pipeline from fastq to methylation reports - BIMSBbioinfo/pigx_bsseq
Running Make: And here is the output of make:rm -rf /home/lindenb/src/ngsxml/OUT/bin/bwa-0.7.10/ && \ mkdir -p /home/lindenb/src/ngsxml/OUT/bin && \ curl -o /home/lindenb/src/ngsxml/OUT/bin/bwa-0.7.10.tar.bz2 -L "http://sourceforge.net… Will download a test_data folder and a configuration file wget https://zerkalo.curie.fr/partage/HiC-Pro/HiCPro_testdata.tar.gz && tar -zxvf HiCPro_testdata.tar.gz ## Edit the configuration file and set the path to Human bowtie2 indexes… $ perl panam2.pl -o example -r1 test/R1.fastq.gz -r2 test/R2.fastq.gz -f Gtgycagcmgccgcggta -r Ccccgycaattcmtttragt -tags test/tags.txt -id 0.97 -dom bacteria -dom archaea -div Y # -div Y corresponds to the script phylodiv_panam.pl For easier use and faster data handling, CRISPRAnalyzeR required gzipped Fastq files (.fastq.gz). Fastq files are usually provided in a gzipped format by the sequencing facility. A toolset for handling sequencing data with unique molecular identifiers (UMIs) - weng-lab/umitools A Fastq quality assessment tool. Contribute to IGBB/quack development by creating an account on GitHub. Quality Control toolkit for Fastq files. Contribute to compbiocore/qckitfastq development by creating an account on GitHub.
Python3 scripts to manipulate Fasta and Fastq files - sanger-pathogens/Fastaq Download the zip file, containing three scripts (merge_sanger_16s.pl, cleanup.pl and subsample_reads.pl) from Coil, Jospin & Lang (2014). { "@id": "/files/Tstff442764/", "@type": [ "file", "item" ], "accession": "Tstff442764", "upload_credentials": { "access_key": "Asiaj2H5Y4GZGL2Trtzq", "expiration": "2015-01-09T10:45:53Z", "federated_user_arn": "arn:aws:sts::618537831167… A project to test my `rnaseq_workflow` repository. Includes rnaseq_workflow as a subtree - russHyde/test_rnaseq_workflow Analysis pipeline for the Guide-seq assay. For news, see: - aryeelab/guideseq
24 Nov 2019 To minimize processing time during testing, each FASTQ file has been and its GFF annotation files (provided in the same download) have been truncated accordingly. data/SRR446039_1.fastq.gz M12A M12 Mock.12h. Defining location of data and establishing basic file structure; Quality trim raw Illumina data using $sample\_trimmed-minlength-$min_length-pe-1.fastq.gz ln -s 5 Sep 2019 We will use ~/test as our working directory, and download fastq files under ~/test/ Fox1_1.fastq.gz, Fox2_1.fastq.gz, and Fox3_1.fastq.gz were 28 Nov 2010 The downloaded files will typically be in gzip (.gz) or gzipped-tar (.tar.gz Assuming a gzipped file called myreads.fastq.gz, the first few lines can to create a file containing a (relatively) small number of reads for initial testing However, the speed of sequencing for these technologies is at the expense of 4.1. gzip. The FASTQ, SAM, BAM files can be compressed also with generic
test_filenames <- c( "./file.fastq ", "file.fq ", "/path/to/file/file.fastq.gz ") modify_filename( test_filenames, new_path = "newdir ") #> [1] "newdir/file.fastq" "newdir/file.fq" "newdir/file.fastq.gz" modify_filename( test_filenames, new…
21 Nov 2019 The R software for running systemPipeR can be downloaded from CRAN. The mini sample FASTQ files used by this overview vignette as well as the associated data/SRR446039_1.fastq.gz M12A M12 Mock.12h. To check if the data is transferred successfully to your account see the step4 of data Data download (Downloading data from users basespace account) Next step is to copy fastq.gz files to local directory, here directory dinosaur which we 20 Jun 2019 This dataset is a random sample of about 5% of the reads from fungal genomic DNA. cd reads. Next, download the fastq.gz files found at Raw sequence file with *.fastq.gz / *.fq.gz suffixed other parameters; NOTE: in lncPipe, test data should be downloaded separately, plz see details in [here](). Available to input gz compressed fastq files (zcat is needed). Please install bowtie2 on your computer and set path to your environment variables. Before running the HLA-HD, check the value of open files on your computer by typing: compression savings for NGS data compared to FASTQ.gz and BAM files. in the cloud to be accessed as if they are local, without downloading them first! of storage costs and transfer times without compromising data quality. Free Trial Filter fastq file based on bed regions. Contribute to stevekm/fastq-bed-subset development by creating an account on GitHub.
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